APC (ACTIVATED PROTEIN C) BIOREAGENTS

For research use only

Human APC

Catalog Numbers: EZ004A (2.5 µg / 1 ml) | EZ004B (10 µg / 1 ml) | EZ004C (100 µg / 1 ml)

Activated with (h) thrombin (in the a form) covalently coupled to agarose beads. Recommended for studies on Protein C-Protein S-Thrombomodulin pathway, and for the Activated Protein C Resistance assay (APC-R), or any research protocol on factors Va and VIIIa.

Stability after Reconstitution > 48hrs.

MW: 60,500 Da.

Inserts: EZ004A | EZ004B | EZ004C

About Purified Human Enzymes (lyophilized)

They are prepared through the specific activation of purified human proteins. Enzymes are very pure and do not contain any activated product (use of solid phase activation, or specific purification following activation). They are then stabilized and lyophilized in presence of the adequate excipients. Following reconstitution, these enzymes present a well characterized activity and a high stability which make them suitable for most of the in vitro biological protocols.

The material used for protein preparation was tested with registered methods and found negative for HIV (1 and 2) antibodies, HBs Ag and HVC antibodies. However, no assay may warrant the total absence of infectious agents. Any product of human origin must then be handled with all the required cautions, as being potentially infectious.

Human APC

Catalog Numbers: EAPC (0.10 mg) | EAPC-1 (1.0 mg)

Activated Protein C (APC) is a serine protease derived from the two chain vitamin K dependent zymogen, Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from homogeneous protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C is homogeneous on 10% SDS-PAGE gels and shows complete reduction upon incubation with 2-mercaptoethanol.

Buffer Composition: 20 mM Tris-HCl/0.1 M NaCl/ pH 7.4

*Extinction Coefficient (1%) = 14.5

Molecular Weight: 56,000 daltons

Bovine APC

Catalog Numbers: EBPCa (0.10 mg) | EBPCa-1 (1.0 mg)

Bovine APC is prepared from homogeneous protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chomatography.